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TK extract induced the mRNA expression of cell proliferation regulatory genes in HaCaT cells. HaCaT cells have been treated With all the indicated concentration of TK extract, and the overall mRNA was used for qRT-PCR.
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While in the wound-therapeutic system, fibroblasts are also significant in creating a new extracellular matrix and collagen framework. In contrast to keratinocytes, fibroblasts didn't demonstrate a substantial boost in proliferation with the treatment of TK extract while in the MTT assay (Determine 5).
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Transfection efficiency for luciferase assay was normalized into the Renilla luciferase exercise. DHT (10 nM) was made use of as being a positive Manage. The bar graphs signify the means ± SD from 3 unbiased experiments.